Longname
Microtubule-affinity-regulating kinase 4
3Letter
MR4
Accession
AK075272
Activation
N/A constitutive
Assay Method
MARK4 (5–20 mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, 1mg/ml BSA) is assayed against KKKVSRSGLYRSPSMPENLNRPR in a final volume of 25.5µl containing 50mM Tris pH 7.5, 0.1 mM EGTA, 0.3mM KKKVSRSGLYRSPSMPENLNRPR, 10 mM magnesium acetate and 0.05mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
3M
Express
Off
ExpressionSys
Sf21 cells
KCode
130
Kinase Type
k
PDF
MARK4_5.pdf
(85.74 KB)
Species
Human
TissRec
R