Protein kinases are the pharmaceutical industry's most important class of drug target. Over 100 protein kinase inhibitors have been approved for clinical use and have had a major impact on the treatment of a number of cancers and other diseases.
The MRC Protein Phosphorylation and Ubiquitylation Unit at The University of Dundee set up the first service to analyse selectivity of protein kinase inhibitors in 1998. This procedure, termed "kinase profiling" proved to be of great help to the pharmaceutical industry, speeding up the development of specific protein kinase inhibitors with therapeutic potential.
The International Centre for Kinase Profiling (ICKP) a key asset to scientists the world over by provides access to kinase profiling services at an affordable price.
Our Approach
We implement the industry level of standard operating procedures that guide all our services. We have provided kinase profiling for many major pharmaceutical companies over the past 30 years including Pfizer, GlaxoSmithKline, Boehringer-Ingelheim, Merck-Serono, Astrazeneca, Janssen Pharmaceutica NV, Novo-Nordisk and Merck & Co.
Quality control measures that we offer include Quantification of Z’ and IC50 values of known kinase inhibitors. The ICKP uses the "gold standard" method for assaying kinases, mainly a radioactive filter binding assay using 33P-ATP (Hastie, et al 2006. Nat Protoc. 2006;1(2):968-71; Bain, et al 2007. Biochem J. 2007 Dec 15;408(3):297-315). This method is sensitive, accurate and provides a direct measure of activity.
We also operate a lipid kinase profiling service as well as a protein kinase profiling service.
Kinase Profiling Inhibitor Database.
The International Centre for Kinase Profiling also incorporates a widely used searchable database of specificities of nearly 250 commonly used signal transduction inhibitors screened against a panel of up to 140 kinases frequently at multiple concentrations. We strongly recommend undertaking profiling of any kinase inhibitor prior to using it as a biological reagent in experiments. To view and peruse data stored in the kinase profiling database click here.
Our Promise
An efficient personalised service for your profiling needs.
If you have a large project to discuss, please contact us and let us help you take your research to the next level!