Longname
c-Jun N-terminal kinase
3Letter
JNK
Accession
L26318
Activation
MKK4 and MKK7
Assay Method
JNK1α1 (5-20 mU diluted in 50 mM Tris pH 7.5, 0.1mM EGTA, 1mg/ml BSA, 0.1% β-mercaptoethanol) is assayed against ATF2 (activating transcription factor in a final volume of 25.5 µl in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, ATF2 (3 µM), 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP] (500 -1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
3
Express
On
ExpressionSys
Sf21 cells
KCode
3
Kinase Type
k
PDF
JNK1_4.pdf
(87 KB)
Species
Human
TissRec
R