Longname
Rho-dependent protein kinase
3Letter
RO2
Accession
U38481
Activation
N/A constitutive
Assay Method
ROCK2 (5-20 mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, 1 mg/ml BSA) is assayed against Long S6 substrate peptide (KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK) in a final volume of 25.5 µl containing 50 mM Tris pH 7.5, 0.1 mM EGTA, 30 µM Long S6 substrate peptide, 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
0J
Express
On
ExpressionSys
Sf21 cells
KCode
19
Kinase Type
k
PDF
ROCK-II_6.pdf
(86 KB)
Species
Rat
TissRec
R