Longname
cyclic AMP-dependent protein kinase
3Letter
PA2
Accession
P17612
Activation
N/A constitutive
Assay Method
PKAα (5-20 mU diluted in 20 mM MOPS pH 7.5, 1 mM EDTA, 0.01% Brij35, 0.1% β-mercaptoethanol, 1 mg/ml BSA) is assayed against Kemptide (LRRASLG) in a final volume of 25.5 µl containing 8 mM MOPS pH 7.5, 0.2 mM EDTA, 30 µM substrate peptide, 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
0W
Express
On
ExpressionSys
E.coli
KCode
32
Kinase Type
k
PDF
PKA_5.pdf
(81.71 KB)
Species
Human
TissRec
R