Longname
Mixed Lineage Kinase 3
3Letter
ML3
Accession
NM_002419
Activation
N/A constitutive
Assay Method
MLK3 (5–20 mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, 1mg/ml BSA) is assayed against MBP in a final volume of 25.5µl containing 50mM Tris pH 7.5, 0.1 mM EGTA, 0.33 mg/ml MBP, 10 mM magnesium acetate and 0.02mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
36
Express
On
ExpressionSys
Sf21 cells
KCode
114
Kinase Type
k
PDF
MLK3_5.pdf
(87.42 KB)
Species
Human
TissRec
R