Longname
Mitogen Activated Protein Kinase Kinase 6 (MAP2K6)
3Letter
MK6
Accession
NM_002758
Activation
c-Raf
Assay Method
MKK6 (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 1 mg/ml BSA) is assayed against a substrate SAPK2a in a final volume of 25.5 µl containing 50mM Tris pH 7.5, 0.1 mM EGTA, 0.25mg/ml SAPK2A, 10 mM magnesium acetate and 0.05 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
45
Express
Off
ExpressionSys
E.coli
KCode
149
Kinase Type
k
PDF
MKK6_5.pdf
(88.91 KB)
Species
Human
TissRec
R