Longname
mitogen-activated protein kinase kinase kinase 1 (MAP3K1)
3Letter
MKK
Accession
XM_042066
Activation
N/A constitutive
Assay Method
MEKK1 (5–20 mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, 1mg/ml BSA) is assayed against MBP in a final volume of 25.5µl containing 50mM Tris pH 7.5, 0.1 mM EGTA, 0.33 mg/ml MBP, 10 mM magnesium acetate and 0.05mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
3S
Express
Off
ExpressionSys
E.coli
KCode
136
Kinase Type
k
PDF
MEKK1_5.pdf
(89.57 KB)
Species
Human
TissRec
R