Longname
MAPK-activated protein kinase 3
3Letter
MK3
Accession
NM_004635
Activation
N/A constitutive
Assay Method
MAPKAP-K3 (5–20 mU diluted in 20 mM MOPS pH 7.5, 1 mM EDTA, 0.01% Brij35, 5% glycerol, 0.1% β-mercaptoethanol, 1mg/ml BSA) is assayed against KKLNRTLSVA in a final volume of 25.5l containing 50 mM Na-β-glycerophosphate pH 7.5, 0.5 mM EDTA, 30 µM substrate peptide, 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
1I
Express
Off
ExpressionSys
E.coli
KCode
54
Kinase Type
k
PDF
MAPKAP-K3_5.pdf
(91.38 KB)
Species
Human
TissRec
R