Longname
MAPK-activated protein kinase-1b
3Letter
RS2
Accession
NM_004586
Activation
N/A constitutive
Assay Method
MAPKAP-K1b/RSK2 (5-20 mU diluted in 20 mM MOPS pH 7.5, 1 mM EDTA, 0.01% Brij35, 5% glycerol, 0.1% β-mercaptoethanol, 1mg/ml BSA) is assayed against substrate peptide (KKLNRTLSVA) in a final volume of 25.5l containing 50 mM Na-b-glycerophosphate (pH 7.5), 0.5 mM EDTA, 30 µM substrate peptide, 10 mM magnesium acetate and 0.05 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
10
Express
Off
ExpressionSys
Sf21 cells
KCode
36
Kinase Type
k
PDF
RSK2_6.pdf
(69.32 KB)
Species
Human
TissRec
R