Longname
eukaryotic translation initiation factor 2-alpha kinase 3
3Letter
EIF
Accession
NM_004836.5
Activation
N/A constitutive
Assay Method
eIF2AK3 (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 10mM DTT, 1 mg/ml BSA) is assayed against a substrate peptide (RSRSRSRSRSRSRSR) in a final volume of 25.5 μl containing 50mM Tris pH 7.5, 0.1mMEGTA, 10mM DTT, 10mM MnCl, 300μM substrate peptide, 10 mM magnesium acetate 0.005 mM [33P-γ-ATP] (50-1000cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 μl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50mM orthophosphoric acid.
ATPGroup
5
Base36
4K
Express
Off
ExpressionSys
E.coli
KCode
164
Kinase Type
k
PDF
eIF2AK3_3.pdf
(88.9 KB)
Species
Human
TissRec
R