Longname
Dual specificity tyrosine phosphorylation Regulated Kinase 2
3Letter
D2A
Accession
NM_003583
Activation
N/A constitutive
Assay Method
DYRK2 (5-20 mU of diluted in 50 mM Tris pH7.5, 0.1 mM EGTA) is assayed against Woodtide (KKISGRLSPIMTEQ) in a final volume of 25.5µl containing 50 mM Tris pH 7.5, 0.1 mM EGTA, 350 µM substrate peptide, 10 mM Magnesium acetate and 0.05 mM [33P-γ-ATP](50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
13
Express
Off
ExpressionSys
E.coli
KCode
39
Kinase Type
k
PDF
DYRK2_3.pdf
(80.28 KB)
Species
Human
TissRec
R