Longname
Dual specificity tyrosine phosphorylation Regulated Kinase 1A
3Letter
DY1
Accession
NM_130437.2
Activation
N/A constitutive
Assay Method
DYRK 1A (5-20 mU of diluted in 50 mM Tris pH7.5, 0.1 mM EGTA) is assayed against Woodtide (KKISGRLSPIMTEQ) in a final volume of 25.5µl containing 50 mM Tris pH 7.5, 0.1 mM EGTA, 350 µM substrate peptide, 10 mM Magnesium acetate and 0.05 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
3A
Express
On
ExpressionSys
E.coli
KCode
118
Kinase Type
k
PDF
DYRK1a_3.pdf
(90.66 KB)
Species
Human
TissRec
R