Longname
C-terminal Src kinase
3Letter
CS1
Accession
NM_004383
Activation
N/A constitutive
Assay Method
CSK (5-20 mU diluted in 20 mM MOPS pH7.5, 1mM EDTA, 0.01% Brij35, 5% glycerol, 0.1% β-mercaptoethanol, 1 mg/ml BSA) is assayed against Cdc2 peptide (KVEKIGEGTYGVVYK) in a final volume of 25.5 µl containing 8 mM MOPS pH7.0, 0.2 mM EDTA, Cdc2 peptide (0.25 mM), 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP](500 -1000 cpm/pmole) and incubated for 30 min at room temperature Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
0P
Express
Off
ExpressionSys
E.coli
KCode
25
Kinase Type
k
PDF
CSK_2.pdf
(94.75 KB)
Species
Human
TissRec
R