Longname
casein kinase-1
3Letter
CKH
Accession
NM_001893
Activation
N/A constitutive
Assay Method
CK1δ (5-20 mU diluted in 20 mM Hepes pH7.5, 0.15 M NaCl, 0.1 mM EGTA, 0.1% Triton X-100, 5 mM DTT, 50% glycerol) is assayed against CKI peptide (RRKDLHDDEEDEAMSITA) in a final volume of 25.5 µl containing 20 mM Hepes pH 7.5, 0.15 M NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% Triton-X 100, CKI peptide (0.5 mM), 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP](500 -1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
3X
Express
On
ExpressionSys
E.coli
KCode
141
Kinase Type
k
PDF
CK1d_2.pdf
(91.26 KB)
Species
Human
TissRec
R