Longname
Choline kinase alpha
3Letter
CHL
Accession
NM_001277.2
Activation
constitutively active
Assay Method
CHK alpha (diluted in 12.5mM Glycine-NaOH (pH 8.5), 50mM KCl, 2.5mM MgCl2, 1mM DTT) is assayed in total volume of 20ul containing 12.5mM Glycine-NaOH (pH 8.5), 50mM KCl, 2.5mM MgCl2, 0.001mM ATPand 0.05mM Choline chloride. The enzyme is assayed for 40 min after which 20ul of ADP-Glo reagent is added. After a further incubation of 40 min, 40ul of Kinase Detection Buffer is added. The assays are incubated for 40 min and then read on PerkinElmer Envision for 1sec/well.
Express
Off
ExpressionSys
bacteria
KCode
108
Kinase Type
l
PDF
CHKA_0.pdf
(85.17 KB)
Species
Human
TissRec
R