Longname
checkpoint kinase-1
3Letter
CH1
Accession
AF016582
Activation
N/A constitutive
Assay Method
CHK1 (5-20 mU diluted in 20 mM MOPS pH 7.5, 1 mM EDTA, 0.1% β-mercaptoethanol, 0.01% Brij-35, 5% glycerol, 1 mg/ml BSA) is assayed against CHKtide substrate peptide (KKKVSRSGLYRSPSMPENLNRPR) in a final volume of 25.5 µl containing 8 mM MOPS pH 7.0, 0.2 mM EDTA, 200 µM CHKtide, 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP](50-1000 cpm/pmole) and incubated for 30 min at room temperature Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
0L
Express
Off
ExpressionSys
Sf21 cells
KCode
21
Kinase Type
k
PDF
CHK1_1.pdf
(86.03 KB)
Species
Human
TissRec
R