Longname
cyclin-dependent kinase 9
3Letter
DK9
Accession
NM_001261 [CDK9] and NM_001240.2 [Cyclin T1]
Activation
N/A constitutive
Assay Method
CDK9/Cyclin T1 (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 1 mg/ml BSA, 0.1% Mercaptoethanol) is assayed against a substrate peptide (YSPTSPSYSPTSPSYSPTSPKKK) in a final volume of 25.5 µl containing 50mM Tris pH 7.5, 0.1mM EDTA, 10mM DTT, 1mg/ml BSA, 0.3mM YSPTSPSYSPTSPSYSPTSPKKK, 10 mM magnesium acetate and 0.05 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
4Y
Express
Off
ExpressionSys
Sf21 cells
KCode
178
Kinase Type
k
PDF
CDK9_0.pdf
(94.32 KB)
Species
Human
TissRec
R