Longname
Abelson murine leukemia viral oncogene homolog 1
3Letter
ABL
Accession
NM_005157
Activation
N/A constitutive
Assay Method
ABL (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 1 mg/ml BSA) is assayed against substrate peptide (EAIYAAPFAKKK) in a final volume of 25.5 μl containing 50mM Tris pH 7.5, 0.1mM EGTA, 1mM DTT , 300μM substrate peptide, 10 mM magnesium acetate and 0.005 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5μl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash bufferof 50 mM orthophosphoric acid.
ATPGroup
5
Base36
4A
Express
Off
ExpressionSys
E.coli
KCode
154
Kinase Type
k
PDF
ABL_screening.pdf
(88.48 KB)
Species
Human
TissRec
R