Longname
With No Lysine deficient protein kinase 1
3Letter
WK1
Accession
NM_018979.3
Activation
Auto-activation
Assay Method
WNK1 (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 1 mg/ml BSA, 0.1% Mercaptoethanol) is assayed against OSR1 in a final volume of 25.5 µl containing 50mM Tris pH 7.5, 0.1mM EDTA, 10mM DTT, 10mM MnCl, 1mg/ml OSR1, 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
5G
Express
Off
ExpressionSys
Sf21 cells
KCode
196
Kinase Type
k
Species
Human
TissRec
R