Longname
Serine / Threonine Kinase 33
3Letter
S33
Accession
BC031231.1
Activation
N/A constitutive
Assay Method
STK33(5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 1 mg/ml BSA0.1%,β-mercaptoethanol) is assayed against asubstrate peptide (AALVRQMSVAFFFKKKKK) in a final volume of 25.5 μl containing 50mM Tris pH 7.5, 0.1mMEGTA,10mM DTT, 300μM substrate peptide, 10 mM magnesium acetate 0.05 mM [33P-γ-ATP] (50-1000cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 μl of0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50mM orthophosphoric acid.
ATPGroup
50
Base36
4E
Express
Off
ExpressionSys
Sf21 cells
KCode
158
Kinase Type
k
PDF
STK33_screening.pdf
(91.71 KB)
Species
Human
TissRec
R