Longname
sarcoma kinase
3Letter
SRC
Accession
NM_005417.3
Activation
N/A constitutive
Assay Method
Src (5–20 mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, 1mg/ml BSA) is assayed against KVEKIGEGTYGVVYK in a final volume of 25.5µl containing 50mM Tris pH 7.5, 0.1mM EGTA, 0.3mM KVEKIGEGTYGVVYK, 10 mM magnesium acetate and 0.05mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
3B
Express
On
ExpressionSys
Sf21 cells
KCode
119
Kinase Type
k
PDF
Src_5.pdf
(82.89 KB)
Species
Human
TissRec
R