Longname
Smooth muscle Myosin Light Chain Kinase
3Letter
ML1
Accession
NM_005965
Activation
N/A constitutive
Assay Method
5-20mU of smMLCK (diluted in 50mM Hepes (pH 7.5), 0.1mM EGTA, 1mg/mlBSA, 0.1%,β-mercaptoethanol) is assayed against substrate peptide (KKRPQRATSNVFA) in a final volume of 25.5µl containing 50mM Hepes (pH 7.5), 0.1mM EGTA, 5mM CaCl2, 10µM Calmodulin, 300µM substrate peptide, 10 mM magnesium acetate and 0.05 mM [33P-γ-ATP]( 500-1000 cpm/pmole) and incubated for 30 mins at room temperature. Assays are stopped by addition of 5µl of 0.5M (3%) orthophosphoric acid. Assays are harvested onto P81 Unifilter plates using a wash buffer of 50mM orthophosphoric acid.
ATPGroup
50
Base36
14
Express
On
ExpressionSys
E. coli
KCode
40
Kinase Type
k
PDF
smMLCK_5.pdf
(77.98 KB)
Species
Human
TissRec
R