Longname
PKC like Kinase 2
3Letter
PR2
Accession
S75548
Activation
N/A constitutive
Assay Method
5-20mU of PRK2 (diluted in 50mM Tris (pH 7.5), 0.1mM EGTA, 1mg/ml BSA, 0.1%,β-mercaptoethanol) is assayed against Long S6 peptide (KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK) in a final volume of 25.5µl containing 50mM Tris (pH 7.5), 0.1mM EGTA, 0.1%, β-mercaptoethanol, 30µM Long S6 peptide, 10 mM magnesium acetate and 0.005 mM [33P-γ-ATP]( 500-1000 cpm/pmole) and incubated for 30 mins at room temperature. Assays are stopped by addition of 5µl of 0.5M (3%) orthophosphoric acid. Assays are harvested onto P81 Unifilter plates using a wash buffer of 50mM orthophosphoric acid.
ATPGroup
5
Base36
16
Express
On
ExpressionSys
Sf21 cells
KCode
42
Kinase Type
k
PDF
PRK2_5.pdf
(79.1 KB)
Species
Human
TissRec
R