Longname
Phosphatidylinositol-5-phosphate 4-kinase type-2 alpha
3Letter
P54
Accession
NM_005028.4
Activation
constitutively active
Assay Method
PIP5K2A ( diluted in 12.5mM Glycine-NaOH (pH 8.5), 50mM KCl, 2.5mM MgCl2, 1mM DTT, 0.25% Na-Cholate and 1mg/ml BSA) is assayed in total volume of 20ul containing 12.5mM Glycine-NaOH (pH 8.5), 50mM KCl, 2.5mM MgCl2, 1mM DTT, 0.25% Na-Cholate, 0.01mM ATPand 0.05mM diC8 PI(5)P. The enzyme is assayed for 15 min after which 20ul of ADP-Glo reagent is added. After a further incubation of 40 min, 40ul of Kinase Detection Buffer is added. The assays are incubated for 40 min and then read on PerkinElmer Envision for 1sec/well.
Express
Off
ExpressionSys
bacteria
KCode
101
Kinase Type
l
PDF
PIP5K2A_5.pdf
(84.69 KB)
Species
Human
TissRec
R