Longname
3-phosphoinositide-dependent protein kinase-1
3Letter
PD1
Accession
AF017995
Activation
N/A constitutive
Assay Method
PDK1 (5-20 mU diluted in 50 mM Tris pH 7.5, 0.05% β-mercaptoethanol, 1 mg/ml BSA) is assayed against PDKtide (KTFCGTPEYLAPEVRREPRILSEEEQ-EMFRDFDYIADWC) in a final volume of 25.5 µl containing 50 mM Tris pH 7.5, 0.05% β-mercaptoethanol, 100 µM substrate peptide, 10mM magnesium acetate and 0.02 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
0E
Express
On
ExpressionSys
Sf21 cells
KCode
14
Kinase Type
k
PDF
PDK1_5.pdf
(96.74 KB)
Species
Human
TissRec
R