Longname
NIMA related Protein Kinase 6
3Letter
NE6
Accession
XM_044814
Activation
N/A constitutive
Assay Method
NEK6 (5-20 mU diluted in 50mM Tris (pH 7.5), 0.1mM EGTA, 1mg/ml BSA, 0.1%,β-mercaptoethanol) is assayed against NEK6 peptide (FLAKSFGSPNRAYKK) in a final volume of 25.5µl containing 50mM Tris (pH 7.5), 0.1mM EGTA, 0.01% Brij, 0.1%, β-mercaptoethanol, NEK6 peptide (0.3 mM), 10 mM magnesium acetate and 0.05 mM [33P-γ-ATP]( 500-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
0T
Express
On
ExpressionSys
Sf21 cells
KCode
29
Kinase Type
k
PDF
NEK6_6.pdf
(88.87 KB)
Species
Human
TissRec
R