Longname
Mammalian sterile 20-like 2 (KRS1 or Ser/Thr protein kinase 3)
3Letter
MT2
Accession
U60206
Activation
N/A constitutive
Assay Method
MST2 (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, 100µM Vanadate) is assayed against MBP in a final volume of 25.5 µl containing 50mM Tris pH 7.5, 0.1mM EGTA, 0.05% β-mercaptoethanol, 0.33mg/ml MBP, 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
1P
Express
On
ExpressionSys
Sf21 cells
KCode
61
Kinase Type
k
PDF
MST2_5.pdf
(76.69 KB)
Species
Human
TissRec
R