Longname
mitogen and stress-activated protein kinase-1
3Letter
MS1
Accession
AF074393
Activation
SAPK2a/p38
Assay Method
MSK1 (5-20 mU diluted in 20 mM MOPS pH 7.5, 1 mM EDTA, 0.01% Brij35, 0.1% β-mercaptoethanol, 1 mg/ml BSA) is assayed against a modified Crosstide peptide GRPRTSSFAEGKK in a final volume of 25.5 µl containing 8 mM MOPS pH7.0, 0.2 mM EDTA, 30 µM substrate peptide, 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
0A
Express
On
ExpressionSys
Sf21 cells
KCode
10
Kinase Type
k
PDF
MSK1_5.pdf
(94.29 KB)
Species
Human
TissRec
R