Longname
glycogen synthase kinase-3-beta
3Letter
G3B
Accession
L33801
Activation
N/A constitutive
Assay Method
GSK3ß (5-20 mU diluted in 20 mM MOPS pH 7.5, 1 mM EDTA, 0.01% Brij35, 5% glycerol, 0.1% β-mercaptoethanol, 1 mg/ml BSA) is assayed against Phospho-GS2 peptide (YRRAAVPPSPSLSRHSSPHQS(PO4)EDEEE) in a final volume of 25.5 µl containing 8 mM MOPS pH 7.0, 0.2 mM EDTA, 20 µM Phospho GS2 peptide, 10 mM magnesium acetate and 0.005 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
5
Base36
OI
Express
On
ExpressionSys
Sf21 cells
KCode
18
Kinase Type
k
PDF
GSK3b_3.pdf
(83.69 KB)
Species
Human
TissRec
R