Longname
Ephrin receptor A4
3Letter
EA4
Accession
NM_004438
Activation
N/A constitutive
Assay Method
EPH-A4 (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 10mM MnCl, 1 mg/ml BSA) is assayed against a substrate peptide (Poly Glut Tyr) in a final volume of 25.5 µl containing 50mM Tris pH 7.5, 0.1mM EGTA, 1mg/ml substrate peptide, 10 mM magnesium acetate and 0.05 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
3P
Express
Off
ExpressionSys
E.coli
KCode
133
Kinase Type
k
PDF
EPHA4_2.pdf
(88.73 KB)
Species
Human
TissRec
R