Longname
Elongation factor kinase
3Letter
EF2
Accession
AAH32665
Activation
N/A constitutive
Assay Method
EF2K (5-20mU diluted in 50 mM Hepes pH 6.6, 0.1% β-mercaptoethanol, 1 mg/ml BSA) is assayed against a substrate peptide (RKKFGESKTKTKEFL) in a final volume of 25.5 µl containing 50mM Hepes pH 6.6, 0.2mM CaCl2, 0.3µM Calmodulin, 0.05% β-mercaptoethanol, 300 µM substrate peptide, 10 mM magnesium acetate and 0.005 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
5
Base36
1N
Express
On
ExpressionSys
E.coli
KCode
59
Kinase Type
k
PDF
EF2K_3.pdf
(79.92 KB)
Species
Human
TissRec
R