Longname
Diacylglycerol kinase gamma
3Letter
DGG
Accession
NM_201532
Activation
constitutively active
Assay Method
DGK gamma i(diluted in 20mM Tris- HCl, 67mM KCl, 10mM MgCl2, 1mM DTT, 0.05mg/ml BSA) is assayed in total volume of 20ul containing 20mM Tris- HCl, 67mM KCl, 10mM MgCl2, 1mM DTT, 0.05mg/ml BSA, 0.01mM ATPand 0.1mM diacyl-glycerol. The enzyme is assayed for 40 min after which 20ul of ADP-Glo reagent is added. After a further incubation of 40 min, 40ul of Kinase Detection Buffer is added. The assays are incubated for 40 min and then read on PerkinElmer Envision for 1sec/well.
Express
Off
ExpressionSys
baculovirus
KCode
139
Kinase Type
l
PDF
DGK_gamma_screening.pdf
(83.25 KB)
Species
Human
TissRec
R