Longname
Death-Associated Protein Kinase 1
3Letter
DP1
Accession
NM_004938.2
Activation
N/A constitutive
Assay Method
DAPK1 (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 1 mg/ml BSA) is assayed against a substrate peptide KKLNRTLSFAEPG) in a final volume of 25.5 µl containing 50mM Tris pH 7.5, 0.1 mM EGTA, 0.3mM peptide, 10mM DTT, 10 mM magnesium acetate and 0.005 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
5
Base36
3Z
Express
Off
ExpressionSys
E.coli
KCode
143
Kinase Type
k
PDF
DAPK1_2.pdf
(90.03 KB)
Species
Human
TissRec
R