Longname
Calmodulin dependent kinase kinase ß
3Letter
CB2
Accession
NM_153499
Activation
N/A constitutive
Assay Method
5-20mU of CaMKKß (diluted in 50mM Tris (pH 7.5), 0.1mM EGTA, 1mg/ml BSA, 0.1%,β-mercaptoethanol) is assayed against substrate peptide (DGEFLRTSCGSPNYAARRR) in a final volume of 25.5µl containing 50mM Tris (pH 7.5), 0.1mM EGTA, 0.5mM CaCl2, 0.3µM calmodulin, 0.1%, β-mercaptoethanol, 300µM substrate peptide, 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP]( 500-1000 cpm/pmole) and incubated for 30 mins at room temperature. Assays are stopped by addition of 5µl of 0.5M (3%) orthophosphoric acid. Assays are harvested onto P81 Unifilter plates using a wash buffer of 50mM orthophosphoric acid.
ATPGroup
20
Base36
1C
Express
On
ExpressionSys
E.coli
KCode
48
Kinase Type
k
PDF
CAMKKb_0.pdf
(84.57 KB)
Species
Human
TissRec
R