Longname
Breast tumour kinase, also know as PTK6 (protein tyrosine kinase 6)
3Letter
BRK
Accession
NM_005975
Activation
N/A constitutive
Assay Method
BRK (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 1 mg/ml BSA0.1%,β-mercaptoethanol) is assayed against asubstrate peptide (Poly Glut Tyr) in a final volume of 25.5 μl containing 50mM Tris pH 7.5, 0.1mMEGTA,10mM DTT, 5mM MnCl, 1mg/ml substrate peptide, 10 mM magnesium acetate 0.02 mM [33P-γ-ATP] (50-1000cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 μl of0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50mM orthophosphoric acid.
ATPGroup
20
Base36
4B
Express
Off
ExpressionSys
Sf21 cells
KCode
155
Kinase Type
k
PDF
BRK_screening.pdf
(84.5 KB)
Species
Human
TissRec
R