Longname
transforming growth factor, beta receptor 1
3Letter
AL5
Accession
NM_004612
Activation
N/A constitutive
Assay Method
TGFβR1/ALK5 (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 5mM MnCl2 1 mg/ml BSA, 0.1% Mercaptoethanol) is assayed against RRKVLTQMGSPSIRCSS*VS in a final volume of 25.5 µl containing 50mM Tris pH 7.5, 0.1mM EDTA, 10mM DTT, 0.3mM substrate, 10 mM magnesium acetate and 0.005 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
59
Express
Off
ExpressionSys
Sf21 cells
KCode
189
Kinase Type
k
PDF
TGFBR1_T204D_screening.pdf
(92.54 KB)
Species
Human
TissRec
R