Longname
platelet-derived growth factor receptor
3Letter
PF4
Accession
NM_006206
Activation
N/A constitutive
Assay Method
PDGFRα (5–20 mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol ,5mM MnCl 1mg/ml BSA, 10mM DTT) is assayed against KKKKEEIYFFFG in a final volume of 25.5µl containing 50mM Tris pH 7.5, 0.1 mM EGTA, 0.3mM KKKKEEIYFFFG, 10 mM magnesium acetate and 0.005mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid
ATPGroup
5
Base36
57
Express
Off
ExpressionSys
Sf21 cells
KCode
187
Kinase Type
k
PDF
PDGFRalpha_%28551-1089%29_screening.pdf
(92.7 KB)
Species
Human
TissRec
R