Longname
testis-specific kinase 1
3Letter
TE1
Accession
NM_006285.2
Activation
N/A constitutive
Assay Method
TESK1 (5–20 mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, 1mg/ml BSA, 10MM DTT) is assayed against Cofilin 2 in a final volume of 25.5µl containing 50mM Tris pH 7.5, 0.1 mM EGTA, 0.2mg/ml Cofilin 2, 10 mM magnesium acetate and 0.05mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
4Q
Express
Off
ExpressionSys
E.coli
KCode
170
Kinase Type
k
PDF
TESK1_5.pdf
(87.87 KB)
Species
Human
TissRec
R