Longname
TANK Binding Kinase 1
3Letter
TBK
Accession
NM_013254
Activation
N/A constitutive
Assay Method
TBK1 (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 1 mg/ml BSA) is assayed against a substrate peptide (KKKKERLLDDRHDSGLDSMKDEE) in a final volume of 25.5 µl containing 50mM Tris pH 7.5, 0.1mM EGTA, 300µM substrate peptide, 10 mM magnesium acetate and 0.05 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
0Y
Express
On
ExpressionSys
Sf21 cells
KCode
34
Kinase Type
k
PDF
GST-TBK1_5.pdf
(88.71 KB)
Species
Human
TissRec
R