Longname
Transforming growth factor beta activated kinase 1 (MAP3K7)
3Letter
TA1
Accession
NM_003188 (TAK1) and NM_006116 (TAB1)
Activation
N/A constitutive
Assay Method
TAK1 (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 1 mg/ml BSA) is assayed against a substrate peptide (RLGRDKYKTLRQIRQ) in a final volume of 25.5 µl containing 50mM Tris pH 7.5, 0.1mM EGTA, 0.1% β-Mercaptoethanol, 300µM substrate peptide, 10 mM magnesium acetate, 0.5mM MnCl and 0.005 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
5
Base36
3Q
Express
On
ExpressionSys
Sf21 cells
KCode
134
Kinase Type
k
PDF
TAK1_5.pdf
(84.58 KB)
Species
Human
TissRec
R