Longname
Serine Arginine Protein Kinase
3Letter
SP1
Accession
NM_003137
Activation
N/A constitutive
Assay Method
5-20mU of SRPK1 (diluted in 50mM Tris (pH 7.5), 0.1mM EGTA, 1mg/ml BSA, 0.1%,β-mercaptoethanol) is assayed against substrate peptide (RSRSRSRSRSRSRSR) in a final volume of 25.5µl containing 50mM Tris (pH 7.5), 0.1mM EGTA, 0.1%, β-mercaptoethanol, 300µM substrate peptide, 10 mM magnesium acetate and 0.05 mM [33P-γ-ATP]( 500-1000 cpm/pmole) and incubated for 30 mins at room temperature. Assays are stopped by addition of 5µl of 0.5M (3%) orthophosphoric acid. Assays are harvested onto P81 Unifilter plates using a wash buffer of 50mM orthophosphoric acid.
ATPGroup
50
Base36
1L
Express
On
ExpressionSys
E.coli
KCode
57
Kinase Type
k
PDF
SRPK1_5.pdf
(75.12 KB)
Species
Human
TissRec
R