Longname
serum and glucocorticoid-induced kinase
3Letter
SG1
Accession
NM_005627
Activation
PDK1
Assay Method
SGK1 (5-20mU diluted in 20 mM MOPS pH 7.5, 1mM EDTA, 0.01% Brij35, 5% glycerol, 0.1% β-mercaptoethanol, 1 mg/ml BSA) is assayed against a modified Crosstide peptide GRPRTSSFAEGKK in a final volume of 25.5 µl containing 8 mM MOPS pH 7.0, 0.2 mM EDTA, 30 µM substrate peptide, 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
0G
Express
On
ExpressionSys
Sf21 cells
KCode
16
Kinase Type
k
PDF
SGK1_5.pdf
(101.62 KB)
Species
Human
TissRec
R