Longname
p38-regulated/activated kinase
3Letter
PR1
Accession
AF032437
Activation
SAPK2a/p38
Assay Method
PRAK/MAPKAPK5 (5-20mU diluted in 50 mM Na-b-glycerophosphate pH 7.5, 0.1mM EGTA, 0.1% β-mercaptoethanol, 1 mg/ml BSA) is assayed against KKLRRTLSVA in a final volume of 25.5 µl containing 50 mM Na-b-glycerophosphate pH 7.5, 0.1 mM EGTA, 30 µM substrate peptide, 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
0B
Express
Off
ExpressionSys
Sf21 cells
KCode
11
Kinase Type
k
PDF
PRAK_5.pdf
(89.43 KB)
Species
Human
TissRec
R