Longname
protein kinase B
3Letter
PBA
Accession
M63167
Activation
PDK1
Assay Method
∆PH-PKBα-S473D (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, 1 mg/ml BSA) is assayed against a modified Crosstide peptide GRPRTSSFAEGKK in a final volume of 25.5 µl containing 50mM Tris pH 7.5, 0.05% β-mercaptoethanol, 30 µM substrate peptide, 10 mM magnesium acetate and 0.005 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
5
Base36
0F
Express
On
ExpressionSys
Sf21 cells
KCode
15
Kinase Type
k
PDF
PKBa_6.pdf
(108.76 KB)
Species
Human
TissRec
R