Longname
protein kinase B ß
3Letter
PBB
Accession
NM_001626
Activation
PDK
Assay Method
∆PH-PKBß (S474D) (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, 1 mg/ml BSA) is assayed against a modified Crosstide peptide (GRPRTSSFAEGKK) in a final volume of 25.5 µl containing 50mM Tris pH 7.5, 0.1mM EGTA, 0.05% β-mercaptoethanol, 30 µM substrate peptide, 10 mM magnesium acetate and 0.05 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
1M
Express
Off
ExpressionSys
Sf21 cells
KCode
58
Kinase Type
k
PDF
PKBb_5.pdf
(85.82 KB)
Species
Human
TissRec
R