Longname
Phosphatidylinositol 4-kinase beta
3Letter
P4B
Accession
BC040300.1
Activation
constitutively active
Assay Method
PIK4CB (diluted in 12.5mM Glycine-NaOH (pH 8.5), 50mM KCl, 2.5mM MgCl2, 1mM DTT, 0.05% CHAPS) is assayed in total volume of 20ul containing 12.5mM Glycine-NaOH (pH 8.5), 50mM KCl, 2.5mM MgCl2, 1mM DTT, 0.05% CHAPS, 0.01mM ATPand 0.25mM natural PI. The enzyme is assayed for 45 min after which 20ul of ADP-Glo reagent is added. After a further incubation of 40 min, 40ul of Kinase Detection Buffer is added. The assays are incubated for 40 min and then read on PerkinElmer Envision for 1sec/well.
Express
Off
ExpressionSys
baculovirus
KCode
122
Kinase Type
l
PDF
PIK4CB_5.pdf
(86.21 KB)
Species
Human
TissRec
R