Longname
stress-activated protein kinase-3
3Letter
SP3
Accession
Y10487
Activation
MKK6DD
Assay Method
p38γ MAPK (5-20 mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1 mM Na3VO4, 0.1% β-mercaptoethanol, 1mg/ml BSA) is assayed against MBP in a final volume of 25.5µl containing 25mM Tris pH 7.5, 0.1 mM EGTA, 0.33 mg/ml MBP, 10 mM magnesium acetate and 0.005mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
5
Base36
6
Express
Off
ExpressionSys
E.coli
KCode
6
Kinase Type
k
PDF
SAPK3_5.pdf
(94.67 KB)
Species
Human
TissRec
R