Longname
stress-activated protein kinase-2α
3Letter
S2A
Accession
L35264
Activation
MKK6DD
Assay Method
p38α MAPK (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1 mM Na3VO4, 0.1% β-mercaptoethanol, 1 mg/ml BSA) is assayed against MBP in a final volume of 25.5 µl containing 25 mM Tris pH 7.5, 0.1 mM EGTA, 0.33 mg/ml MBP, 10 mM magnesium acetate and 0.05 mM [33P-γ-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
4
Express
On
ExpressionSys
E.coli
KCode
4
Kinase Type
k
PDF
SAPK2b_5.pdf
(94.39 KB)
Species
Human
TissRec
R