Longname
NIMA related Protein Kinase 2a
3Letter
NE2
Accession
NM_002479
Activation
N/A constitutive
Assay Method
5-20mU of NEK2a (diluted in 50mM Tris (pH 7.5), 0.1mM EGTA, 1mg/ml BSA, 0.1%,β-mercaptoethanol) is assayed against NEK2a peptide (RFRRSRRMI) in a final volume of 25.5µl containing 50mM Tris (pH 7.5), 0.1mM EGTA, 0.01% Brij, 0.1%, β-mercaptoethanol, 300µM NEK2a peptide, 10 mM magnesium acetate and 0.05 mM [33P-γ-ATP]( 500-1000 cpm/pmole) and incubated for 30 mins at room temperature. Assays are stopped by addition of 5µl of 0.5M (3%) orthophosphoric acid. Assays are harvested onto P81 Unifilter plates using a wash buffer of 50mM orthophosphoric acid.
ATPGroup
50
Base36
0U
Express
Off
ExpressionSys
Sf21 cells
KCode
30
Kinase Type
k
PDF
NEK2a_5.pdf
(72.9 KB)
Species
Human
TissRec
R